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Drosophila media

I have been using the following instant dry media, without significant alterations, to culture Drosophila hydei and D. melanogaster for about 6 years now. It’s a popular formula that has been posted widely online, but typically in volume units only. Below is my conversion to grams, which I find more convenient when making large batches.

This formula can be easily scaled up or down as needed. Multiply by 0.75 to make a batch that fills a gallon freezer bag. Multiply by 4.5 to make a batch that fills a 5-gallon pail.

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